M7.1: Zytokin-Gentransfer in Tumor- und Effektorzellen zur Verbesserung der körpereigenen Immunabwehr bei Leukämie

Dokumentation 1997–2006

Projektleiter:

Dr. phil.nat. Michaela Scherr

Abt. Hämatologie und Onkologie
Medizinische Hochschule Hannover
Carl-Neuberg-Str. 1
30625 Hannover 

PD Dr. med. Ulrike Blömer

Abt. für Neurochirurgie
Medizinische Hochschule Hannover
Carl-Neuberg-Str. 1
30625 Hannover

Projekttitel:

Lentiviraler Gentransfer von Ribozymen/Antisense-RNA gegen BCR-ABL und die gemeinsame ?-Kette der Rezeptoren für Interleukin-3 und GM- CSF in hämatopoetische Stamm- und Vorläuferzellen bei der chronisch myeloischen Leukämie (CML). Präklinische Untersuchungen zur Effizienz einer Suppression von Onkogen- und Zytokin-Rezeptor-Funktionen in CML-Zellen.

Projektdauer:

9/1999 - 10/2002

Veröffentlichungen:

• M. Scherr, K. Battmer, U. Blömer, A. Ganser, M. Grez
  Quantitative determination of lentiviral vector particle numbers by realtime PCR
  Bio Techniques (2000) Vol 31 (3)
• K. Wagner, S. Kafert-Kasting, G. Heil, A. Ganser, M. Eder
  Inhibition of granulocyte-macrophage colony-stimulating factor receptor function by a splice variant of the common beta-receptor subunit
  Blood 2001; 98: 2687-2694
• M. Scherr, J. LeBon, D. Castanotto, H. C. Schrieb, P. S. Meltzer, A. Ganser, A. D. Riggs, J. J. Rossi
  Detection of antisense and ribozyme accessible sites on native messenger RNAs: Application to Amplified in Breast cancer-1 (AIB-1) mRNA
  Molecular Therapy
• M. Scherr, K. Battmer, M. Eder, S. Schüle, H. Hohenberg, A. Ganser, M. Grez, U. Blömer
  Efficient gene transfer into the CNS by lentiviral vectors purified by anion exchange chromatography
• M. Scherr, K. Battmer, U. Blömer, B. Schiedlmeier, A. Ganser, M. Grez, M. Eder
  Lentiviral gene transfer into peripheral blood-derived CD34 + NOD/SCID-repopulating cells
  Blood (2002) Volume 99, Number 2
• Ch. Demaison, K. Parsley, G. Brouns, M. Scherr, K. Battmer, Ch. Kinnon, M. Grez, A. J. Thrasher
  High-level transduction and gene expression in hematopoietic repopulating Cells using a human imunodeficiency virus typ 1-based letiviral vector containing an interna spleen focus forming virus promoter
  Human Gene Therapy 13: 803-813 (May 1,2002)
• M. Scherr, M. Eder
  Gene transfer into hematopoietic stem cells using lentiviral vectors
  Current Gene Therapy 2002,2 (45 ? 55)
• M. Scherr, K. Battmer, Th. Winkler, O. Heidenreich, A. Ganser, M. Eder
  Specific inhibition of bcr-abl gene expression by small interfering RNA
  Blood, 15 February 2003, Volume 101, Number 4
• M. Scherr, M. A. Morgan, M. Eder
  Gene silencing mediated by small interfering RNAs in mammalian cells
  Current Medicinal Chemistry 2003, 10 (245 ? 256)
• M. Scherr, K. Battmer, A. Ganser, M. Eder
  Modulation of gene expression by lentiviral-mediated delivery of small interfering RNA
  Landes Biosciende; Cell Cycle (2003) Vol. 2 Issue 3

 

M7.2: Zytokin-Gentransfer in Tumor- und Effektorzellen zur Verbesserung der körpereigenen Immunabwehr bei Leukämie

Projektleiter:

Prof. Dr. Andreas Neubauer

Abteilung für Innere Medizin
SP Hämatologie/Onkologie/Immunologie
Klinikum der Philipps-Universität Marburg
Baldingerstraße
35043 Marburg

Projekttitel:

Transfer Interferon-regulatorischer Gene in Zellen der normalen und malignen Hämatopoese

Projektdauer:

10/1999 - 10/2002

Veröffentlichungen:

• S. A. Koenig-Merediz, M. Schmidt, G. J. Hoppe, J. Alfken, D. Meraro, B.-Z. Levi, A. Neubauer, B. Wittig
  Cloning of an interferon regulatory factor 2 isoform with different regulatory ability
  Nucleic Acids Research (2000); Vo. 28, No. 21: 4219-4224
• M. Schmidt, A. Hochhaus, S. A. König-Merediz, C. Brendel, J. Proba, G. J. Hoppe, B. Wittig, G. Ehninger, R. Hehlmann, A. Neubauer
  Expression of Interferon Regulatory Factor 4 in Chronic Myeloid Leukemia: Correlation with response to interferon alfa therapy
  Journal of Clinical Oncology, Vol. 18, No. 19 (October 1), 2000: pp 3331-3338
• M. Schmidt, A. Hochhaus, A. Nitsche, R. Hehlmann, A. Neubauer
  Expression of nuclear transcription factor ICSBP in CML correlates with pre-treatment risk features and cytogenetic response to interferon-alpha
  Blood, 15 MAY 2001; Vol. 97, No.10
• A.Burchert. S. Wölfl, M. Schmidt,, C. Brendel, B. Denecke, D. Cai, L. Odyvanova, T. Lahaye, M. C. Müller, T. Berg, H. Gschaidmeier, B. Wittig, R. Hehlmann, A. Hochhaus, A. Neubauer
  Interferon-?, but not the ABL-kinase inhibitor imatinib (STI571), induces expression of myeloblastin and a specific T-cell response
  Blood, 1 January 2003, Volume 101, Number 1
• M. Schmidt, A. Hochhaus, A. Nitsche, R. Hehlmann, A. Neubauer
  Expression of nuclear transcription factor interferon consensus sequence binding protein in chronic myeloid leukemia correlates with pretreatment risk features and cytogenetic response to interferon-alpha
  Blood, 1 June 2001, Volume 97, Number 11
• S. A. König Merediz, M. Schmidt, G. J. Hoppe, J. Alfken, D. Meraro, B.-Z. Levi, A. Neubauer, B. Wittig
  Cloning of an interferon regulatory factor 2 isoform with different regulatory ability
  Nucleic Acids Researchs, 2000, Vol. 28, No. 21, 4219-4224
• M. Schmidt, A. Hochhaus, S. A. König-Merediz, C. Brendel, J. Proba, G. J. Hoppe, B. Wittig, G. Ehninger, R. Hehlmann, A. Neubauer
  Expression of interferon regulatory factor 4 in chronic myeloid leukemia: Correlation with response to interferon alfa therapy
  Journal of Clinical Oncology, Vol 18, Nov. 19 (October 1), 2000: pp 3331-3338
• J.Köchling, S.A.König-Merediz, R.Stripcke, D.Buchwald, A.Korte, H.G. von Einsiedel, F.Sack, G. Henze, K. Seeger, B. Wittig, M.Schmidt
  Protection of mice against Philadelphia Chromosome-positive acute Lymphoblastic Leukemia by Cell-based Vaccination using nonviral, minimalistic expression vectors and immunomodulatory oligonucleotides
  Clinical Cancer Research, (2003) Vol.9, 3142-3149
• Manuel Schmidt, Christina A. Friedrich, Andreas Burchert, Katharina Hölzl, Andreas Nitsche, Burghardt Wittig, A.Neubauer
  Down-regulation of interferon regulatory factor 4 gene expression in leukemic cell lines is due to hypermethylation of CpG sites in the promoter region
  Dali Cai, Andreas Burchert, Christina A. Friedrich, Justus Duyster, Özkan Alkasi, Rolf Müller, Andreas Neubauer, Manuel Schmidt
  IRF4 decreases tumorigenicity of bcr-abl-transformed 32D cells in vivo and reverses the bcr-abl-mediated phenotype
• Andreas Burchert, Dali Cai, Lorenz Hofbauer, Magnus Samuelsson, Emily P.Slater, Justus Duyster, Markus Ritter, Andreas Hochhaus, Rolf Müller, Martin Eilers, Manuel Schmidt, A.Neubauer
  Interferon consensus sequence binding protein (ICSBP, IRF-8) antagonizes BCR/ABL and down-regulates bcl-2
  BLOOD, 1May 2004, Vol. 103, Number 9

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